anti α 2c antibody  (Bioss)

Journal: eLife

Article Title: Dysfunctional TRPM8 signalling in the vascular response to environmental cold in ageing

doi: 10.7554/eLife.70153

Figure Lengend Snippet: blood flow in hindpaw with intraplantar injection of noradrenaline (1.25 ng/µl in saline in 20 µl) in young and aged mice (n = 8). ( b ) % maximum change in blood flow from baseline induced by noradrenaline (maximum vasoconstriction). ( c ) Graph shows the mean ± s.e.m. blood flow in hindpaw with intraplantar injection of medetomidine (1.25 ng/µl in saline in 20 µl) in young and aged mice (n = 8–10) ( d ) % maximum change in blood flow from baseline induced by medetomidine (maximum vasoconstriction). ( e ) Representative western blot of alpha2C (α 2c ) adrenoceptor in mice hindpaw skin with densitometric analysis normalised to GAPDH. ( f ) Representative western blot of tyrosine hydroxylase (TH) in mice hindpaw skin with densitometric analysis normalised to GAPDH (Y = young, A = aged). (n = 7) *p < 0.05, **p < 0.01. (Two-tailed Student’s t-test).

Article Snippet: The primary antibodies were made in 3 % PBST solution at 1:500 dilution for TRPM8 (Alomone Labs #ACC049, RRID: AB_2040254 ), 1:1,000 dilution for α 2c adrenergic receptor (Bio-Techne #NB100-93554, RRID: AB_1236641 ), phospho TH (Bio-Techne #NB300-173, RRID: AB_2201648 ), total TH (Bio-Techne #NB300-109, RRID: AB_10077691 ), p21 (Santa Cruz #sc-6246, RRID: AB_628073 ).

Techniques: Injection, Saline, Western Blot, Two Tailed Test

Journal: eLife

Article Title: Dysfunctional TRPM8 signalling in the vascular response to environmental cold in ageing

doi: 10.7554/eLife.70153

Figure Lengend Snippet: ( a–c ) RT-PCR CT analysis shows fold change of α 2a adrenoceptor , α 2c adrenoceptor and Trpv1 in young and aged mice normalised to three housekeeping genes in (DRG). Results are shown as mean ± s.e.m. (n = 14–16) *p < 0.05, ***p < 0.001. (Two-tailed Student’s t-test).

Article Snippet: The primary antibodies were made in 3 % PBST solution at 1:500 dilution for TRPM8 (Alomone Labs #ACC049, RRID: AB_2040254 ), 1:1,000 dilution for α 2c adrenergic receptor (Bio-Techne #NB100-93554, RRID: AB_1236641 ), phospho TH (Bio-Techne #NB300-173, RRID: AB_2201648 ), total TH (Bio-Techne #NB300-109, RRID: AB_10077691 ), p21 (Santa Cruz #sc-6246, RRID: AB_628073 ).

Techniques: Reverse Transcription Polymerase Chain Reaction, Two Tailed Test

Journal: eLife

Article Title: Dysfunctional TRPM8 signalling in the vascular response to environmental cold in ageing

doi: 10.7554/eLife.70153

Figure Lengend Snippet: The local cold exposure produces rapid vasoconstriction which is significantly blunted in the aged mice (see blood flow graph at top centre). Cold water (4 °C) exposure to hindpaw activates the cold receptors TRPA1 and TRPM8 in sympathetic nerves, which leads to increased intracellular calcium and release of NA. This signalling, however, is significantly downregulated in aged mice due to diminished expression of TRPA1/TRPM8 in sympathetic nerves. NA acts on the post-synaptic α 2c adrenergic receptors on smooth muscle cells to mediate vasoconstriction. However, α 2c adrenergic receptor are also significantly diminished in aged mice, which leads to reduced signalling. All these factors contribute to an attenuated vascular cold response in aged mice compared to young mice.

Article Snippet: The primary antibodies were made in 3 % PBST solution at 1:500 dilution for TRPM8 (Alomone Labs #ACC049, RRID: AB_2040254 ), 1:1,000 dilution for α 2c adrenergic receptor (Bio-Techne #NB100-93554, RRID: AB_1236641 ), phospho TH (Bio-Techne #NB300-173, RRID: AB_2201648 ), total TH (Bio-Techne #NB300-109, RRID: AB_10077691 ), p21 (Santa Cruz #sc-6246, RRID: AB_628073 ).

Techniques: Expressing

Journal: eLife

Article Title: Dysfunctional TRPM8 signalling in the vascular response to environmental cold in ageing

doi: 10.7554/eLife.70153

Figure Lengend Snippet:

Article Snippet: The primary antibodies were made in 3 % PBST solution at 1:500 dilution for TRPM8 (Alomone Labs #ACC049, RRID: AB_2040254 ), 1:1,000 dilution for α 2c adrenergic receptor (Bio-Techne #NB100-93554, RRID: AB_1236641 ), phospho TH (Bio-Techne #NB300-173, RRID: AB_2201648 ), total TH (Bio-Techne #NB300-109, RRID: AB_10077691 ), p21 (Santa Cruz #sc-6246, RRID: AB_628073 ).

Techniques:

Journal: The Journal of Veterinary Medical Science

Article Title: Inhibition of transient receptor potential vanilloid type 1 through α 2 adrenergic receptors at peripheral nerve terminals relieves pain

doi: 10.1292/jvms.21-0429

Figure Lengend Snippet: Sequences of primers for RT-PCR

Article Snippet: Subsequently, the sections were incubated with a guinea pig anti-TRPV1 antibody (Merck Millipore corporation, Temecula, CA, USA, Cat# AB5566, Lot# 3135959) diluted to 1:50, a rabbit anti-α 2A antibody (Cusabio Technology, Houston, TX, USA, Cat# CSB-PA007424, Lot# G1229Y) diluted to 1:100 or a rabbit anti-α 2C antibody (Cusabio Technology, Cat# CSB-PA000916, Lot# G1229Y) diluted to 1:100 overnight at 4°C.

Techniques:

Journal: The Journal of Veterinary Medical Science

Article Title: Inhibition of transient receptor potential vanilloid type 1 through α 2 adrenergic receptors at peripheral nerve terminals relieves pain

doi: 10.1292/jvms.21-0429

Figure Lengend Snippet: Subtypes of the α 2 adrenergic receptors detected in dorsal root ganglia. A. The real-time PCR amplification curves are shown in the left panel. A threshold was set for 3, and the Ct values of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and α 2 receptors were obtained. The ΔCt values of α 2A (n=8), α 2B (n=8) and α 2C (n=8) receptors against GAPDH were calculated and are shown in the right panel. The columns represent the mean ± the standard error of the mean. B, C. Images of the immunoreactivities of TRPV1, α 2A and α 2C receptors in rat dorsal root ganglia. The TRPV1-, α 2A receptor- and α 2C receptor-like immunoreactivities were visualized by 3,3′-diaminobenzidine. Arrows, positively stained small-sized neurons; arrowheads, positively stained medium-sized neurons.

Article Snippet: Subsequently, the sections were incubated with a guinea pig anti-TRPV1 antibody (Merck Millipore corporation, Temecula, CA, USA, Cat# AB5566, Lot# 3135959) diluted to 1:50, a rabbit anti-α 2A antibody (Cusabio Technology, Houston, TX, USA, Cat# CSB-PA007424, Lot# G1229Y) diluted to 1:100 or a rabbit anti-α 2C antibody (Cusabio Technology, Cat# CSB-PA000916, Lot# G1229Y) diluted to 1:100 overnight at 4°C.

Techniques: Real-time Polymerase Chain Reaction, Amplification, Staining